Recombinant Enterokinase Specifications
| Product Name | Recombinant Enterokinase |
| Source | Recombinant E. coli |
| Appearance | Colorless to light yellow clear liquid |
| Activity | ≥5.0 U/μl |
| Purity(SDS-PAGE) | Single major band 25.8±2.6kDa |
| Storage Conditions | -15°C below，protected from light, sealed. |
The data should be subject to test report of products.
Recombinant Enterokinase Application
Enzyme digestion condition: 25mM Tris-HCl 8.0, fusion protein concentration: 0.1-1mg/ml (total
protein content: 50-100μg), EK content: 0.1-2U, Temperature: 25℃, overnight or 16h-24h for
digestion.
4°C low temperature digestion conditions: 4°C
can effectively digest the substrate, but need to
extend the digestion time to 48-64h, or increase
the enzyme 2-3 times.
Enzyme cleavage optimization and amplification:
Optimization: Enzyme cleavage conditions can be
optimized, such as buffer pH, fusion protein
concentration, EK amount, and digestion time, so
that the fusion protein can be digested under stable
conditions, SDS-PAGE detection of digestion
effect. Amplification: Select the optimized
reaction conditions, according to the conditions
such as the proportion of amplification, SDS- PAGE detection of digestion effect.
＞200mM imidazole or ＞200mM NaCl or ＞5%
glycerin, the reaction may be effected.The
following suggestions are given: To receive the
optimum result, please dialyze the sample to 25
mMTris-HCl, pH 8.0. If the dialysis is
inconvenient, please dilute the sample to ＜
100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of fusion protein and EK may
not be changed (1U:500μg fusion protein). If there
are one or more components in samples, and
cannot be removed, suggest to increase the content
of EK in reaction system or extend the reaction
time.
Enterokinase Enzyme Transport and Storage Stability
Stability of storage: Recombinant Enterokinase should be stored at -15°C below in sealed container.
It is stable within 24 months.If stored at 25°C ,it is stable within one week.
It is no activity loss after 5 times repeated freezing and thawing.
Stability of transport: The product is stable by ice insulation transport.
Notice and Disclaimer
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
